Crystal Structure of the glycogen phosphorylase B / N-(beta-D-glucopyranosyl)-N'-cyclopropyl oxalamide complex

N-(BETA-D-GLUCOPYRANOSYL)-N'-CYCLOPROPYL OXALAMIDE; N-[(cyclopropylamino)(oxo)acetyl]-beta-D-glucosylamine; N-[(cyclopropylamino)(oxo)acetyl]-D-glucosylamine; N-[(cyclopropylamino)(oxo)acetyl]-glucosylamine

Binding of oxalyl derivatives of beta-d-glucopyranosylamine to muscle glycogen phosphorylase b.

SRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPK
RIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIR
YEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNT
MRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRD
PVRTNFDAFPDKVAIQLNDTHPSLAIPELMRVLVDLERLDWDKAWEVTVKTCAYTNHTVLPEALERWPVHLLETLLPRHL
QIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQ
NKTNGITPRRWLVLCNPGLAEIIAERIGEEYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPN
SLFDVQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFVVPRTVMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPVVGDRL
RVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDR
LDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYVKCQERVSALYKNPREWTRM
VIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKIP

10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K

The same complex with N-(beta-d-glucopyranosyl)oxamic acid

The same complex with methyl-N-(beta-D-glucopyranosyl)oxamate

The same complex with ethyl-N-(beta-D-glucopyranosyl)oxamate

Dimeric glycogen phosphorylase is the physiologically active species

glycogenolysis, type 2 diabetes, TRANSFERASE