Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP

Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.

The pilus-retraction protein PilT: ultrastructure of the biological assembly

MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEKHRQKLEENG
QVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFKKLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYI
NQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNTAIRNLIRENKLQQVYSLMQS
GQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELERMIRGGRHHHHHH

5-8 mg/ml protein
30-45% MPD
0.2-0.4 M Ammonium Sulfate
Tris, 15mM
KCl 75mM
5% glycerol
5-10mM magnesium Chloride
1-5 mM ATPgammaS

, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

The biological assembly is a hexamer generated from the monomer in the asymmetric unit by all six operations in P6.

Pilus Retraction Motor, ATPase, Hexameric PilT, PROTEIN TRANSPORT

MFEKQEVEQKKELKILEIIKEAIELGASDIHLTAGAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEKHRQKLEENG
QVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFKKLGLPDKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYI
NQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGT
LHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGGGRVLAYELLIPNTAIRNLIRENKLQQVYSLMQS
GQAETGMQTMNQTLYKLYKQGLITLEDAMEASPDPKELERMIRGGR