Crystal Structure of the (S)-Specific 1-Phenylethanol Dehydrogenase of the Denitrifying Bacterium Strain EbN1

Crystal Structure and Enzyme kinetics of the (S)-Specific 1-Phenylethanol Dehydrogenase of the denitrifying bacterium Strain EbN1

MTQRLKDKLAVITGGANGIGRAIAERFAVEGADIAIADLVPAPEAEAAIRNLGRRVLTVKCDVSQPGDVEAFGKQVISTF
GRCDILVNNAGIYPLIPFDELTFEQWKKTFEINVDSGFLMAKAFVPGMKRNGWGRIINLTSTTYWLKIEAYTHYISTKAA
NIGFTRALASDLGKDGITVNAIAPSLVRTATTEASALSAMFDVLPNMLQAIPRLQVPLDLTGAAAFLASDDASFITGQTL
AVDGGMVRH

12-18% PEG 8000, 0.05M potassium phasphate, 3% 2-methyl-2,4-pentanediol crystal was soaked with 5mM NAD+ and 5mM (R)-1-phenylethanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K

the biological assembly is the tetramer generated by a two fold axis