Solution Structure of Human Sorting Nexin 22 PX Domain
Center for Eukaryotic Structural Genomics (CESG)
Solution structure of human sorting nexin 22.
GHHHHHHLELEVHIPSVGPEAEGPRQSPEKSHMVFRVEVLCSGRRHTVPRRYSEFHALHKRIKKLYKVPDFPSKRLPNWR TRGLEQRRQGLEAYIQGILYLNQEVPKELLEFLRLRHFPTDPKASNWG
WHEAT GERM CELL-FREE, IN VITRO EXPRESSION
Center for Eukaryotic Structural Genomics
ALL TRIPLE-RESONANCE AND NOESY SPECTRA WERE ACQUIRED USING A CRYOGENIC PROBE.
STRUCTURES ARE BASED ON A TOTAL OF 1885 NOE RESTRAINTS (801 INTRA, 449 SEQUENTIAL, 277 MEDIUM, AND 358 LONG RANGE), 76 HBOND RESTRAINTS, AND 172 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS.
TORSION ANGLE DYNAMICS FOR INITIAL STRUCTURE CALCULATION, MOLECULAR DYNAMICS, SIMULATED ANNEALING FOR FINAL STRUCTURE REFINEMENT
0.5 MM 13C,15N-LABELED BC019655, 10 MM BIS-TRIS, 100 MM NACL, 10 MM DTT, 50 MM ARGININE HYDROCHLORIDE, 50 MM SODIUM GLUTAMATE, 90% H2O, 10% D2O
G. Marius Clore, John Kuszewski, Charles D. Schwieters, Nico Tjandra.
PX domain, sorting nexin 22, BC019655, SNX22_HUMAN, Hs.157607, STRUCTURAL GENOMICS, PROTEIN STRUCTURE INITIATIVE, PSI, CENTER FOR EUKARYOTIC STRUCTURAL GENOMICS, CESG, Protein Transport
LEVHIPSVGPEAEGPRQSPEKSHMVFRVEVLCSGRRHTVPRRYSEFHALHKRIKKLYKVPDFPSKRLPNWRTRGLEQRRQ GLEAYIQGILYLNQEVPKELLEFLRLRHFPTDPKASNWG