Crystal Structure Of Biotin Protein Ligase From Aquifex Aeolicus

RIKEN Structural Genomics/Proteomics Initiative (RSGI)

(MSE)FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGRLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLS
VSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLK
VLKRISENLKKFKEKSFKEFKGKIESK(MSE)LYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRR
S

MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGRLGRKWLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEA
LEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKR
ISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEILSGEFSLRRS

27.5w/v(%) PEG 4K, 0.1M Hepes-NaOH, pH 7.4, microbatch, temperature 295K

RIKEN Structural Genomics/Proteomics Initiative
NPPSFA, National Project on Protein Structural and Functional Analyses

The biological unit is a dimer and identical to the asymmetric unit

Biotin Biosynthesis, Dimer, Structural Genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Ligase