TNF Receptor Subtype One-selective TNF Mutant with Antagonistic Activity

Creation and X-ray structure analysis of the tumor necrosis factor receptor-1-selective mutant of a tumor necrosis factor-alpha antagonist

VRSSSRTPSDMPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFSGQGCPSTHVLLTHTI
SRISTTHNQPVNLLSAIRSPCQRETPEGAEANPWYEPIYLGGVFQLEPGDRLSAEINRPDYLDFAESGQVYFGIIAL

This mutant was created by phage display system

0.05M HEPES pH7.5, 1.5%(w/v) 1,2,3-Heptanetriol, 12.5% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Tumor necrosis factor, Trimer, antagonistic activity, TNFR1 specific, phage display system, CYTOKINE

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTI
SRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL