Solution structure of the GUCT domain from human ATP-dependent RNA helicase DDX50, DEAD box protein 50
RIKEN Structural Genomics/Proteomics Initiative (RSGI)
Solution structure of the GUCT domain from human RNA helicase II/Gubeta reveals the RRM fold, but implausible RNA interactions
DEAD box protein 50, Nucleolar protein Gu2, Gu-beta
GSSGSSGFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAE WHDSDWILSVPA
RIKEN Structural Genomics/Proteomics Initiative
NPPSFA, National Project on Protein Structural and Functional Analyses
HNN and CACO RDC data, obtained from the Pf1 phage system, were used in the torsion angle dynamics calculation.
torsion angle dynamics, restrained molecular dynamics
0.9mM protein U-15N,13C; 20mM d-Tris-HCl(pH7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O, 90% H2O
ATP binding, Helicase, Hydrolase, Nuclear protein, Nucleotide-binding, RNA-binding, GUCT domain, Structural Genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI
FEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWI LSVPA