Fully automated NMR structure determination of the ENTH-VHS domain AT3G16270 from Arabidopsis thaliana
Automated Protein Structure Determination from NMR Spectra
NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana
GSSGSSGESYWRSRMIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLDNKSPIVKQKALRLIKYAVGKS GSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRETAHETISAIFSEENGSGPSSG
structure of the same protein determined by conventional nmr methods
structures with the least restraint violations
Fully automated NMR spectrum analysis and structure calculation without human intervention. Chemical shift assignments and conformational restraints have not been verified manually.
1.20MM 13C, 15N-ARABIDOPSIS ENTH-VHS DOMAIN; 20MM TRIS BUFFER; 100MM NACL; 1MM DITHIOTHREITOL; 0.02% NA3N; 90% H2O, 10% D2O
Fully automated structure determination; FLYA algorithm, UNKNOWN FUNCTION