Double layered 2D crystal structure of AQUAPORIN-4 (AQP4M23) at 3.2 a resolution by electron crystallography

Implications of the Aquaporin-4 Structure on Array Formation and Cell Adhesion

data was collected by electron microscope equipped with a helium stage

AQP-4, WCH4, Mercurial-insensitive water channel, MIWC

MVAFKGVWTQAFWKAVTAEFLAMLIFVLLSVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTV
AMVCTRKISIAKSVFYITAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKR
TDVTGSVALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGALYEYVFCPDVELKRRL
KEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGDEKKGKDSSGEVLSSV

10mM MES(pH 6.0), 100mM NaCl, 50mM MgCl2, 2mM DTT, 1% glycerol, dialysys, temperature 293K

EXPERIMENT TYPE : ELECTRON DIFFRACTION
DATE OF DATA COLLECTION : 01-APR-2002
TEMPERATURE (KELVIN) : 4.2
PH : 6.00
NUMBER OF CRYSTALS USED : 135
RADIATION SOURCE : JEM3000SFF
OPTICS : CRYSTALS TILTED TO MAX
60 DEGREES
DETECTOR TYPE : CCD
DETECTOR MANUFACTURER : GATAN ULTRASCAN
INTENSITY INTEGRATION SOFTWARE : PICKYCOR, AN MRC ELECTRON
DIFFRACTION PROGRAM
DATA SCALING SOFTWARE : MERGEDIFF,AN MRC ELECTRON
DIFFRACTION PROGRAM
ACCELERATION VOLTAGE (KV) : 300
NUMBER OF UNIQUE REFLECTIONS : 5992
RESOLUTION RANGE HIGH (A) : 3.20
RESOLUTION RANGE LOW (A) : 22.21
OVERALL.
COMPLETENESS FOR RANGE (%) : 87.0
DATA REDUNDANCY : NULL
R MERGE (I) : 0.223
R SYM (I) : NULL
<I/SIGMA(I)> FOR THE DATA SET : NULL

IN THE HIGHEST RESOLUTION SHELL.
HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.20
HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 3.40
COMPLETENESS FOR SHELL (%) : 85.8
DATA REDUNDANCY IN SHELL : NULL
R MERGE FOR SHELL (I) : 0.445
R SYM FOR SHELL (I) : NULL
<I/SIGMA(I)> FOR SHELL : NULL

METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR
REPLACEMENT
SOFTWARE USED: CNS
STARTING MODEL: PDB ENTRY 1J4N

WATER TRANSPORT, WATER CHANNEL, AQUAPORIN, TWO-DIMENSIONAL CRYSTAL, MEMBRANE PROTEIN, BACULOVIRUS EXPRESSION SYSTEM, TRANSPORT PROTEIN