Structural analysis of membrane-bound mastoparan-X by solid-state NMR
Structure of tightly membrane-bound mastoparan-x, a g-protein-activating Peptide, determined by solid-state NMR.
G protein-bound conformation of mastoparan-X
Solution structure analysis of the mastoparan with detergents
Signal assignments and chemical-shift structural analysis of uniformly 13C, 15N-labeled peptide, mastoparan-X, by multidimensional solid-state NMR under magic-angle spinning
The structure was determined using triple-resonance 1H/13C/15N and double-resonance 1H/13C probes with 4mm and 3.2mm spinner modules.
the structures are based on a total of 31 restraints, 5 distance restraints derived by RR and REDOR experiments and 26 dihedral angle restraints predicted by TALOS.
2mg or 5mg uniformly 13C, 15N labeled MP-X bound to membrane: Molar ratio MP-X:membrane=1:20
membrane DPPC:DPPG=4:1 relative humidity 32%
2mg selectively 13C labeled MP-X bound to membrane: Molar ratio MP-X:membrane=1:20
2mg selectively 13C, 15N labeled MP-X bound to membrane: Molar ratio MP-X:membrane=1:20
MASTOPARAN X, MP-X, membrane bound, Mast cell degranulation, Venom, Amidation, TOXIN