Refinement of RNase P P4 stemloop structure using residual dipolar coupling data, C70U mutant cobalt(III) hexammine complex
Solution Structure and Metal-Ion Binding of the P4 Element from Bacterial Rnase P RNA
5'-R(*GP*GP*AP*AP*GP*UP*UP*CP*CP*GP *UP*CP*UP*UP*CP*GP*GP*AP*CP*CP*GP*GP*CP*UP*UP*CP*C)-3'
REFINEMENT OF P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLING DATA
REFINEMENT OF RNASE P P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLING DATA - C70U MUTANT
REFINEMENT OF RNASE P P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLINGS - COBALT( III) HEXAMMINE COMPLEX STRUCTURE
THE SEQUENCE CORRESPONDS TO NUCLEOTIDES 66 TO 73, AND 354 TO 360 OF THE E. COLI RNASE P RNA, WITH TWO G:C PAIRS ADDED ON THE 5' SIDE OF THE HELIX, AND ONE U:A PAIR, ONE C:G PAIR AND A UUCG LOOP ADDED ON THE OTHER SIDE. IN THE PDB ENTRY,NUCLEOTIDES 3-10 CORRESPOND TO 66-73 IN E. COLI RNASE P RNA, AND NUCLEOTIDES 19-25 CORRESPOND TO 334-360.
THE STRUCTURE WAS DETERMINED USING CONSTRAINTS FROM HOMONUCLEAR NMR EXPERIMENTS AS DESCRIBED IN ENTRY 17F9, AND ADDITIONAL RESIDUAL DIPOLAR COUPLINGS FOR IMINO RESONANCES AQCUIRED WITH AND WITHOUT 30 MG PER ML PF1 PHAGE PRESENT IN SOLUTION
DETAILS OF THE REFINEMENT PROCEDURE AGAINST RESIDUAL DIPOLAR COUPLINGS ARE GIVEN IN THE PRIMARY CITATION ABOVE
C70U MUTANT, COBALT (III HEXAMMINE COMPLEX, METAL BINDING SITE, METAL COMPLEX, P4 STEM, RIBONUCLEASE P, RIBONUCLEIC ACID, RIBOZYME, TRANSFER RNA PROCESSING, NUCLEIC ACID