Refinement of P4 stemloop structure using residual dipolar coupling data
Solution Structure and Metal-Ion Binding of the P4 Element from Bacterial Rnase P RNA
5'-R(*GP*GP*AP*AP*GP*UP*CP*CP*GP*GP*UP*CP*UP *UP*CP*GP*GP*AP*CP*CP*GP*GP*CP*UP*UP*CP*C)-3'
REFINEMENT OF RNASE P P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLING DATA - C70U MUTANT
REFINEMENT OF RNASE P P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLINGS - COBALT( III) HEXAMMINE COMPLEX STRUCTURE
REFINEMENT OF RNASE P P4 STEMLOOP STRUCTURE USING RESIDUAL DIPOLAR COUPLING DATA, C70U MUTANT COBALT(III) HEXAMMINE COMPLEX
THE SEQUENCE CORRESPONDS TO NUCLEOTIDES 66 TO 73, AND 354 TO 360 OF THE E. COLI RNASE P RNA, WITH TWO G:C PAIRS ADDED ON THE 5' SIDE OF THE HELIX, AND ONE U:A PAIR, ONE C:G PAIR AND A UUCG LOOP ADDED ON THE OTHER SIDE. IN THE PDB ENTRY,NUCLEOTIDES 3-10 CORRESPOND TO 66-73 IN E. COLI RNASE P RNA, AND NUCLEOTIDES 19-25 CORRESPOND TO 334-360.
THE STRUCTURE WAS DETERMINED USING CONSTRAINT DATA FROM HOMONUCLEAR NMR SPECTROSCOPY AS DESCRIBED IN ENTRY 1F6X, AND ADDITIONAL RESIDUAL DIPOLAR COUPLINS FOR THE IMINO RESONANCES DERIVED FROM HSCQ TYPE EXPERIMENTS ACQUIRED IN THE PRESENCE AND ABSENCE OF 22 MG PER ML PF1 PHAGE
DETAILS OF THE REFINEMENT PROCEDURE AGAINST RESIDUAL DIPOLAR COUPLINGS ARE GIVEN IN THE PRIMARY REFERENCE CITATION ABOVE
METAL BINDING SITE, P4 STEM, RIBONUCLEASE P, RIBONUCLEIC ACID, RIBOZYME, TRANSFER RNA PROCESSING, NUCLEIC ACID