Lectin from Ralstonia solanacearum complexed with Me-fucoside

ALPHA-L-METHYL-FUCOSE; methyl 6-deoxy-alpha-L-galactopyranoside; methyl alpha-L-fucoside; methyl L-fucoside; methyl fucoside

The fucose-binding lectin from Ralstonia solanacearum. A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.

SSVQTAATSWGTVPSIRVYTANNGKITERCWDGKGWYTGAFNEPGDNVSVTSWLVGSAIHIRVYASTGTTTTEWCWDGNG
WTKGAYTSTN

HANGING DROPS WITH 2.5 UL RSL AT 15MG/ML, 2 UL OF RESERVOIR SOLUTION (30% PEG 6000, 0.1M TRIS/HCL PH 8.2) AND 1 UL OF GLYCINE.

LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH 2-FUCOSYLLACTOSE

LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH XYLOGLUCAN FRAGMENT

FIRST METHIONINE NOT IN THE MATURE PROTEIN. THE CONFLICTS IN THE SEQADV RECORDS SHOWN BELOW ARISE BECAUSE THIS PROTEIN IS FROM A DIFFERENT STRAIN OF RALSTONIA SOLANACEARUM.

DISORDERED REGIONS WERE MODELED STEREOCHEMICALLY. OCCUPANCIES OF SIDE CHAINS MODELLED IN ALTERNATIVE POSITIONS WERE ESTIMATED USING RELATIVE B-FACTORS. OCCUPANCY OF THE S-ME GROUP OF METHIONINE RESIDUES WAS ESTIMATED USING DIFFERENCE MAP RESIDUALS. THESE RESIDUES HAD CLEARLY SUFFERED RADIATION DAMAGE AND HAD NO ALTERNATIVE CONFIGURATION. TOTAL NUMBER OF BINS

LECTIN, SUGAR RECOGNITION, BETA-PROPELLER