high resolution structure of GDP-bound human Rab4a

High Resolution Crystal Structures of Human Rab4A in its Active and Inactive Conformations.

NUCLEOTIDE-BINDING DOMAIN, RESIDUES 1-184

GHMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYY
RGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENV
EEAFVQCARKILNKIESGELDPERMG

PARTIALLY REFINED GPPNHP-BOUND RAB4A STRUCTURE WAS USED FOR MOLECULAR REPLACEMENT, SOLVED BY SAME AUTHORS

30% (W/V) PEG 8000, 200MM AMMONIUM SULPHATE, 100MM SODIUM CACODYLATE PH6.5 HANGING DROP 20 DEGREE CELSIUS

HIGH RESOLUTION STRUCTURE OF GPPNHP-BOUND HUMAN RAB4A

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGIONS WERE MODELED STEREOCHEMICALLY

GTP-BINDING PROTEIN, VESICULAR TRANSPORT, ENDOCYTOSIS, PRENYLATION, PROTEIN TRANSPORT, TRANSPORT