Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH7 using 20mM ZnSO4 in buffer. 1mM DTT was used as a reducing agent

Effect of Ph on the Active Site of an Arg121Cys Mutant of the Metallo-Beta-Lactamase from Bacillus Cereus: Implications for the Enzyme Mechanism

Exploring the Role and the Binding Affinity of a Second Zinc Equivalent in B. Cereus Metallo-Beta-Lactamase

Crystal Structure of the Zinc-Dependent Beta-Lactamase from Bacillus Cereus at 1.9A Resolution: Binuclear Active Site with Features of a Mononuclear Enzyme

METALLO-BETA-LACTAMASE, PENICILLINASE, CEPHALOSPORINASE

SQKVEKTVIKNETGTISISQLNKNVWVHTELGSFNGEAVPSNGLVLNTSKGLVLVDSSWDDKLTKELIEMVEKKFQKRVT
DVIITHAHADCIGGIKTLKERGIKAHSTALTAELAKKNGYEEPLGDLQTVTNLKFGNMKVETFYPGKGHTEDNIVVWLPQ
YNILVGGCLVKSTSAKDLGNVADAYVNEWSTSIENVLKRYRNINAVVPGHGEVGDKGLLLHTLDLLK

PROTEIN WAS CRYSTALLISED USING HANGING DROP VAPOR DIFFUSION. RESERVOIR SOLUTION CONTAINED 100MM TRIS AT PH4.5-5, 70-75% AMMONIUM SULPHATE, 1MM DTT OR 1MM DTT AND 1MM TCEP-HCL, 2MM ZNSO4 AND 0.1% AZIDE. PROTEIN CONCENTRATION OF 2.7 MG/ML. DROPS WERE KEPT AT 291K AND WERE STREAK SEEDED FROM A WILD TYPE CRYSTAL AFTER 1 DAY., pH 7.00

ZN-DEPENDENT METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS

STRUCTURE OF A ZINC METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS

METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS 569/H/9

METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS 569/H/9 C168S MUTANT

METALLO-BETA-LACTAMASE BCII CD SUBSTITUTED FROM BACILLUS CEREUS AT 1.35 ANGSTROMS RESOLUTION

METALLO BETA-LACTAMASE II FROM BACILLUS CEREUS 569/H/9 AT PH 6.0, TRIGONAL CRYSTAL FORM

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH5 USING 20MM ZNSO4 IN BUFFER. 1MM DTT WAS USED AS A REDUCING AGENT.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH4.5 USING 20MM ZNSO4 IN BUFFER. 1MM DTT WAS USED AS A REDUCING AGENT. CYS221 IS OXIDIZED.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH4.5 USING 20MM ZNSO4 IN THE BUFFER. 1MM DTT AND 1MM TCEP-HCL WERE USED AS REDUCING AGENTS. CYS221 IS REDUCED.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH5 USING 20 MICROMOLAR ZNSO4 IN THE BUFFER. 1MM DTT WAS USED AS A REDUCING AGENT. CYS221 IS OXIDIZED.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH4.5 USING 20 MICROMOLAR ZNSO4 IN THE BUFFER. 1MM DTT WAS USED AS A REDUCING AGENT. CYS221 IS OXIDIZED.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH4.5 USING 20 MICROMOLAR ZNSO4 IN THE BUFFER. 1MM DTT AND 1MM TCEP-HCL WERE USED AS REDUCING AGENTS.

BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII ) ARG (121) CYS MUTANT. SOLVED AT PH7 USING 20 MICROMOLAR ZNSO4 IN THE BUFFER. 1MM DTT WAS USED AS A REDUCING AGENT. CYS221 IS OXIDIZED.

METALLO BETA-LACTAMASE II FROM BACILLUS CEREUS 569/H/9 AT PH 6.0, MONOCLINIC CRYSTAL FORM

ENGINEERED RESIDUE IN CHAIN A, CYS 121 ARG ENGINEERED RESIDUE IN CHAIN B, CYS 121 ARG BETA-LACTAM + H2O = SUBSTITUTED BETA-AMINO ACID.
THE SEQUENCE NUMBERING USED IN THIS ENTRY IS BASED ON A STANDARD NUMBERING SYSTEM DEVISED TO ALLOW FOR EASY COMPARISON BETWEEN DIFFERENT MEMBERS OF THE BACILLUS CEREUS METALLO-BETA-LACTAMASE FAMILY. SEE ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, MAR. 2001, VOL 45, P660-663

HYDROLASE, ZINC, METALLO-BETA-LACTAMASE, ANTIBIOTIC RESISTANCE