Solution structure of a multifunctional DNA- and protein-binding domain of human Werner syndrome protein
Solution structure of a multifunctional DNA- and protein-binding motif of human Werner syndrome protein.
Chirality error at the CG center of Leu A 115
MDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFL VEVSRYNKFMKICALTKKGRNWLHKANTESQSLILQANEELCPKKLLLPSSKTVSSGTKEHCYN
HCCH-COSY was optimized for detection for CH3 groups, 2D spin echo for 1H-13C HSQC selected for aromatic and Carbonyl containing residues
back calculated data agree with experimental NOESY spectrum,structures with acceptable covalent geometry
simulated annealing torsion angle dynamics
0.35 mM in KPi buffer (pH7.4) 25 mM NaCl
Schwieters, C.D., Kuszewski, J., Tjandra, N. & Clore, G.M. (2003)
the WH-like domain, DNA BINDING PROTEIN, PROTEIN BINDING