solution structure of the theta subunit of escherichia coli DNA polymerase III in complex with the epsilon subunit
Structure of the theta subunit of Escherichia coli DNA polymerase III in complex with the epsilon subunit
THE STRUCTURE WAS DETERMINED USING DISTANCE AND ANGULAR RESTRAINTS AND SUPPLEMENTED WITH RESTRAINTS CALCULATED FROM PSEUDO-CONTACT SHIFTS INDUCED BY INSERTING A LANTHANIDE ION INTO THE EPSILON186 BINDING POCKET
THE STRUCTURES ARE BASED ON 632 DISTANCE RESTRAINTS, 111 DIHEDRAL ANGLE RESTRAINTS AND 162 PSEUDO-CONTACT SHIFT RESTRAINTS. THE PSEUDO-CONTACT SHIFTS WERE CALCULATED FROM THE CHANGE IN CHEMICAL SHIFT OF THE AMIDE PROTONS INDUCED BY INSERTING A LANTHANIDE ION (EITHER YSPROSIUM, ERBIUM OR HOLMIUM) AT THE METAL BINDING SITE OF EPSILON-186 WHICH IS COMPLEXED TO THE THETA SUBUNIT. XPLOR-NIH 2.9.7 WAS USED TO REFINE THE STRUCTURE USING THIS DATA
1MM THETA-EPSILON186 U15N,13C(THETA); 100mM NaCl, 0.1mM DITHIOTHREITOL; 20mM PHOSPHATE BUFFER Na; 90% H20, 10% D2O
1MM THETA-EPSILON186 U15N,13C(THETA); 100mM NaCl, 0.1mM DITHIOTHREITOL; 20mM TRIS BUFFER; 90% H20, 10% D2O
C.BARTELS, T.XIA, M.BILLETER, P.GUENTERT, K.WUETHRICH
C.SCHWIETERS, N.TJANDRA, J.KUSZEWSKI, G.CLORE
THETA SUBUNIT, DNA POLYMERASE III, TRANSFERASE