Structure of the Escherichia coli quorum sensing protein SdiA: activation of the folding switch by acyl homoserine lactones.
MSDKDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSLCVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAID PVLNPENFSQGHLMWNDDLFSEAQPLWEAARAHGLRRGVTQYLMLPERALGFLSFSRCSAREIPILSDELQLKMQLLVRE SLMALMRLNDE
torsion angle dynamics simulated annealing
0.2 mM 13C, 15N labeled protein,50mM sodium acetic, 2mM DTT, 1mM EDTA, 0.2M Urea,pH 4.2, H2O
0.2 mM 13C, 15N labeled protein,50mM sodium acetic, 2mM DTT, 1mM EDTA, 0.2M Urea,pH 4.2, D2O
0.3 mM 13C, 15N, 2H labeled protein,50mM sodium acetic, 2mM DTT, 1mM EDTA, 0.2M Urea,pH 4.2, H2O
homoserine lactone, quorum sensing, TRANSCRIPTION