Structure of MurF from Streptococcus pneumoniae co-crystallized with a small molecule inhibitor exhibits interdomain closure

UDP-N-acetylmuramoylalanine-D-glutamyl-lysine-D-alanyl-D-alanine ligase, MurF protein

(MSE)KLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHP
YILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKD(MSE)LAHLLSTRYKTYKTQGNYNNEIGLPYTVLH(MSE)
PEGTEKLVLE(MSE)GQDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGK(MSE)QIADG(MSE)ASGSLLL
APADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNA(MSE)IASYVALQEGVSE
EQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTA(MSE)KLILETFSAIPANEGGKKIAVLAD(MSE)KELGDQS
VQLHNQ(MSE)ILSLSPDVLDIVIFYGEDIAQLAQLASQ(MSE)FPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQIL
LKGSNS(MSE)NLAKLVESLEN

MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILV
DDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAPADPIVEDYLPIDKKVVRFGQGA
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADI
LSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQLAQLASQM
FPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLEN

Crystal structure of sp protein ligand 1