Purine nucleoside phosphorylase from calf spleen

(2S,4R,6R,6AS)-4-(2-AMINO-6-OXO-1,6-DIHYDROPURIN-9-YL)-6-(HYDROXYMETHYL)-TETRAHYDROFURO[3,4-D][1,3]DIOXOL-2-YLPHOSPHONI C ACID

Novel multisubstrate inhibitors of mammalian purine nucleoside phosphorylase.

Calf Spleen Purine Nucleoside Phosphorylase Complexed with Substrates and Substrate Analogs.

MQNGYTYEDYQDTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSEIPNFPESTVPGHAGRLVFGILNGRACVM
MQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFP
AMSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSL
ITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPVSGHTG

calf spleen purine nucleoside phosphorylase purchased from Sigma Co.

PEG2K MME , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K

The biological assembly is a trimer generated from the monomer in the asymmetric unit.

purine nucleoside phosphorylase, multisubstrate analog inhibitor, transferase