2.5A X-ray Structure of Engineered OspA protein

Atomic structures of peptide self-assembly mimics.

Design of
single-layer beta-sheets without a hydrophobic core

Engineered Outer Surface Protein A (OspA) with the inserted two beta-hairpins

GSHMKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVF
KEDGKTLVSKKVTSKDKSSTEEKFNEKGELSEKKITRADKSSTEEKFNEKGELSEKKITRADKSSTEEKFNEKGEVSEKI
ITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAW
NSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

SINGLE-LAYER BETA-SHEET, BETA-HAIRPIN, REPEAT PROTEIN, De Novo Protein, Immune System

NSVSVDLPGEMNVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVFKEDGK
TLVSKKVTSKDKSSTEEKFNEKGE

VSEKIITRADGTRLEYTEIKSDGSGKAKEVLKSYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATK
KTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK