Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins
Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins.
Designed zinc finger protein by Phage display
This protein has the same sequence as ZNF29G29R with the terminal glycine replaced by an arginine.
SEQUENCE THE SEQUENCE HAS NOT BEEN DEPOSITED IN ANY DATABASE
structures with the least restraint violations
The structure is based on a total of 242 restraints, 177 are NOE-derived distance restraints, 41 dihedral angle restraints, 16 distance restraints from hydrogen bonds, 8 distance constraints from coordinated Zinc ion.
simulated annealing, torsion angle dynamics, molecular dynamics
1.2 mM ZNF29G29R; 10 mM phosphate buffer pH 6.8, 2mM Mercaptoethanol, 200 uM Sodium Azide, 50 uM Zinc Sulfate, 90% H2O, 10% D2O
1.2 mM ZNF29G29R; 10 mM phosphate buffer pH *6.4, 2mM Mercaptoethanol, 200 uM Sodium Azide, 50 uM Zinc Sulfate,"100%" D2O
1.2 mM ZNF29G29R 15N; 10 mM phosphate buffer pH 6.8, 2mM Mercaptoethanol, 200 uM Sodium Azide, 50 uM Zinc Sulfate, 90% H2O, 10% D2O
zinc finger protein, beta beta alpha, RREIIB-TR, RNA BINDING PROTEIN