THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT
RESIDUES PRO 39 AND PRO 55 ARE CIS PROLINES.
Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant.
ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPKKYNNYEGFDFSVSSPYYEWPILSSGDVYSGGSPGADRVVF NENNQLAGVITHTGASGNNFVECT
THE PROTEIN USED IN THIS STUDY IS THE ISOZYME OF RIBONUCLEASE T1 WHERE A LYSINE RESIDUE REPLACES GLUTAMINE 25 OF THE SEQUENCED RIBONUCLEASE T1 (K.TAKAHASHI, J.BIOCHEM.,V. 98, P. 815, 1985).
RIBONUCLEASE T1 (E.C.3.1.27.3) MUTANT WITH HIS 40 REPLACED BY LYS (H40K) COMPLEX WITH PHOSPHATE
MMYSKLLTLTTLLLPTALALPSLVERACDYTCGSNCYSSSDVSTAQAAGYQLHEDGETVGSNSYPHKYNNYEGFDFSVSS PYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNFVECT