TAR RNA recognition by a cyclic peptidomimetic of Tat protein
RNA was transcribed in vitro from DNA oligonucleotide templates with T7 RNA polymerase purified in house with unlabeled or 13C/15N enriched NTPs (Silantes) and purified by denaturing PAGE.
The BIV-2 peptide was synthesized by solid phase synthesis and purified by reverse phase HPLC
structures with the least restraint violations
0.8 mM BIV-2 peptide plus unlabeled 0.8 mM BIV TAR RNA, 20 mM Sodium Acetate, D3, pH 5.0, 100% D2O
20 mM Sodium Acetate, D3, pH 5.0, 100% D2O
0.8 mM BIV-2 peptide plus unlabeled 0.8 mM BIV TAR RNA, 20 mM Sodium Acetate, D3, pH 5.0, 90% H2O, 10% D2O
20 mM Sodium Acetate, D3, pH 5.0, 90% H2O, 10% D2O
0.6 mM BIV-2 peptide plus 13C/15N labeled 0.6 mM BIV TAR RNA, 20 mM Sodium Acetate, D3, pH 5.0, 100% D2O
0.6 mM BIV-2 peptide plus 13C/15N labeled 0.6 mM BIV TAR RNA, 20 mM Sodium Acetate, D3, pH 5.0, 90% H2O, 10% D2O
NMR; peptidomimetics; peptide structure, RNA recognition; immunodeficiency virus; TAR RNA;, RNA binding protein-RNA COMPLEX