Solution structure of a self-sacrificing resistance protein, CalC from Micromonospora echinospora

Center for Eukaryotic Structural Genomics (CESG)

Structural insight into the self-sacrifice mechanism of enediyne resistance.

Resistance to enediyne antitumor antibiotics by CalC self-sacrifice.

The calicheamicin gene cluster and its iterative type I enediyne PKS.

NYDPFVRHSVTVKADRKTAFKTFLEGFPEWWPNNFRTTKVGAPLGVDKKGGRWYEIDEQGEEHTFGLIRKVDEPDTLVIG
WRLNGFGRIDPDNSSEFTVTFVADGQKKTRVDVEHTHFDRMGTKHAKRVRNGMDKGWPTILQSFQDKIDEEGAKK

Center for Eukaryotic Structural Genomics

The structures are based on a total of 3042 restraints, 2766 are NOE-derived distance constraints, 176 dihedral angle restraints,100 distance restraints from hydrogen bonds.

1mM CalC U-15N,13C; 10mM phosphate buffer, 150 mM NaCl, 90% H2O, 10% D2O

Self-Sacrificing Resistance Protein, Structural Genomics, PSI, Protein Structure Initiative, Center for Eukaryotic Structural Genomics, CESG, TOXIN