Structure of the globular head domain of the bundlin, BfpA, of the bundle-forming pilus of Enteropathogenic E.coli
Structure of the Bundle-forming Pilus from Enteropathogenic Escherichia coli
Complete resonance assignments of bundlin (BfpA) from the bundle-forming pilus of enteropathogenic Escherichia coli
Major structural subunit of bundle-forming pilus
MEQSASDSNKSQNAISEVMSATSAINGLYIGQTSYSGLDSTILLNTSAIPDNYKDTTNKKITNPFGGELNVGPANNNTAF GYYLTLTRLDKAACVSLATLNLGTSAKGYGVNISGENNITSFGNSADQAAKSTAITPAEAATACKNTDSTNKVTYFMK
structures with acceptable covalent geometry, structures with the least restraint violations, structures with the lowest energy
577 Ambiguous and 2277 unambiguous NOE restraints, 86 TALOS constraints and 38 hydrogen bonds were imposed during ARIA structure calculations.
Simulated annealing with NMR restraints using ARIA
least number of violations, lowest energy
0.5 mM BfpA U-15N,13C, 20 mM sodium phosphate buffer pH 5.2, 90%H20, 10% D2O
Major structural subunit of bundle-forming pilus precursor
alpha-beta fold, beta-sandwich, one disulfide bond, CELL ADHESION
QSASDSNKSQNAISEVMSATSAINGLYIGQTSYSGLDSTILLNTSAIPDNYKDTTNKKITNPFGGELNVGPANNNTAFGY YLTLTRLDKAACVSLATLNLGTSAKGYGVNISGENNITSFGNSADQAAKSTAITPAEAATACKNTDSTNKVTYFMK