Catalytic and ubiqutin-associated domains of MARK2/PAR-1: K82R mutant

Structure of the catalytic and ubiquitin-associated domains of the protein kinase MARK/Par-1.

MAP/Microtubule affinity regulating kinase 2
CATALYTIC AND UBIQUITIN-ASSOCIATED DOMAINS

PAR-1; Kin1; serine/threonine kinase Emk; serine/threonine-protein kinase MARK2; ELKL Motif Kinase; EMK1

GNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVRIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFE
VIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFS
NEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDC
ENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMAT
YLLLGYK

PEG3350, Ammonium Sulphate, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

T208A/S212A inactive double mutant with selenomethionine

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

SERINE/THREONINE PROTEIN KINASE; MARK; PAR-1; KIN1; UBA DOMAIN, SIGNALING PROTEIN, TRANSFERASE

NSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEV
IETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSN
EFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCE
NLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATY
LLLGYK