How sequence defines structure: a crystallographic map of DNA structure and conformation.
Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.00
DNA WAS SYNTHESIZED ON AN APPLIED BIOSYSTEMS DNA SYNTHESIZER USING PHOSPHORAMIDITE CHEMISTRY, WITH THE TRITYL-PROTECTING GROUP LEFT INTACT AT THE 5'-TERMINAL NUCLEOTIDE THEN DEPROTECTED BY TREATMENT WITH 3% ACETIC ACID FOR FIFTEEN MINUTES, NEUTRALIZED WITH AMMONIUM HYDROXIDE, AND DESALTED ON A SIGMA G-25 SEPHADEX COLUMN.
STRUCTURE NOT REFINED TO ITS LOWEST R AND RFREE VALUES. PLEASE REFER TO CITATION ABOVE FOR MORE DETAILS.
Crystallographic Screen, DNA Structure, Holliday Junction, Molecular Structure, DNA