How sequence defines structure: a crystallographic map of DNA structure and conformation.
STRUCTURE IS NOT REFINED TO ITS LOWEST R AND RFREE VALUES, REFER TO LITERATURE CITATION FOR THIS STRUCTURE.
NA CACODYLATE, CACL2, SPERMINE, MPD, IN RESEVOIR,, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K
DNA WAS SYNTHESIZED ON AN APPLIED BIOSYSTEMS DNA SYNTHESIZER USING PHOSPHORAMIDITE CHEMISTRY, WITH THE TRITYL-PROTECTING GROUP LEFT INTACT AT THE 5'-TERMINAL NUCLEOTIDE THEN DEPROTECTED BY TREATMENT WITH 3% ACETIC ACID FOR FIFTEEN MINUTES, NEUTRALIZED WITH AMMONIUM HYDROXIDE, AND DESALTED ON A SIGMA G-25 SEPHADEX COLUMN.
THIS STRUCTURE IS NOT FULLY REFINED. PART OF A CRYSTALLOGRAPHIC SCREEN - SEE REFERENCE.
CRYSTALLOGRAPHIC SCREEN, DNA STRUCTURE, HOLLIDAY JUNCTION, MOLECULAR STRUCTURE, DNA