DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation
MAKKLLPAFQNAERLLLAHMMRSRDVALVVQERIGGRFNIEEHRALAAYIYAFYEEGHEADPGALISRIPGELQPLASEL SLLLIADDVSEQELEDYIRHVLNRPKWLMLKVKEQEKTEAERRKDFLTAARIAKEMIEMKKMLSSS
SEQUENCE According to authors, residue 530 in the actual sequence is a Glu and 531 is a Leu. The initial sequence submitted in the database for these residues is incorrect.
simultaneously acquired 15N- and 13C-edited 3D NOESY
20mM potassium phosphate, 200mM sodium chloride
total of 1707 experimentally determined restraints, 1439 NOE-derived distance constraints, 200 dihedral angle restraints, 68 hydrogen bond distance restraints
torsion angle dynamics followed by cartesian slow-cool annealing energy minimisation
two alpha helical sub-domains, TRANSFERASE
MLAKKLLPAFQNAERLLLAHMMRSRDVALVVQERIGGRFNIEEHRALAAYIYAFYEEGHEADPGALISRIPGELQPLASD VSLLLIADDVSEQELEDYIRHVLNRPKWLMLKVKEQEKTEAERRKDFLTAARIAKEMIEMKKMLSSS