Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute virus of Mice

Structure Determination of Minute Virus of Mice

MSDGTSQPDGGNAVHSAARVERAADGPGGSGGGGSGGGGVGVSTGSYDNQTHYRFLGDGWVEITALATRLVHLNMPKSEN
YCRIRVHNTTDTSVKGNMAKDDAHEQIWTPWSLVDANAWGVWLQPSDWQYICNTMSQLNLVSLDQEIFNVVLKTVTEQDS
GGQAIKIYNNDLTACMMVAVDSNNILPYTPAANSMETLGFYPWKPTIASPYRYYFCVDRDLSVTYENQEGTIEHNVMGTP
KGMNSQFFTIENTQQITLLRTGDEFATGTYYFDTNPVKLTHTWQTNRQLGQPPLLSTFPEADTDAGTLTAQGSRHGATQM
EVNWVSEAIRTRPAQVGFCQPHNDFEASRAGPFAAPKVPADVTQGMDREANGSVRYSYGKQHGENWAAHGPAPERYTWDE
TNFGSGRDTRDGFIQSAPLVVPPPLNGILTNANPIGTKNDIHFSNVFNSYGPLTTFSHPSPVYPQGQIWDKELDLEHKPR
LHITAPFVCKNNAPGQMLVRLGPNLTDQYDPNGATLSRIVTYGTFFWKGKLTMRAKLRANTTWNPVYQVSVEDNGNSYMS
VTKWLPTATGNMQSVPLITRPVARNTY

10mM Tris-HCl, 8 mM CaCl2, PEG8000, virus 10mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP

Coordinates and associated matrices have been transformed from the icosahedral point symmetry frame to the crystallographic frame

SEQUENCE
THE AUTHORS VERIFIED WITH THEIR COLLABORATORS
THAT THE THIS DIFFERENCE BETWEEN THE CRYSTAL
COORDINATES AND SEQUENCE DATABASE IS DUE TO
AN ERROR IN THE DEPOSITED SEQUENCE OR A
POSSIBLE DIFFERENCE IN AN EARLIER VARIANT OF
THE VIRUS. RESIDUE 366 IS A METHONINE AND
THE RESIDUE 455 IS A THEORNINE.

icosahedral asymmetric unit, std point frame

Immunosuppressive strain, MVMi, Minute virus of Mice, Icosahedral virus, Virus-DNA COMPLEX

MSDGTSQPDGGNAVHSAARVERAADGPGGSGGGGSGGGGVGVSTGSYDNQTHYRFLGDGWVEITALATRLVHLNMPKSEN
YCRIRVHNTTDTSVKGNMAKDDAHEQIWTPWSLVDANAWGVWLQPSDWQYICNTMSQLNLVSLDQEIFNVVLKTVTEQDS
GGQAIKIYNNDLTACMMVAVDSNNILPYTPAANSMETLGFYPWKPTIASPYRYYFCVDRDLSVTYENQEGTIEHNVMGTP
KGMNSQFFTIENTQQITLLRTGDEFATGTYYFDTNPVKLTHTWQTNRQLGQPPLLSTFPEADTDAGTLTAQGSRHGATQM
EVNWVSEAIRTRPAQVGFCQPHNDFEASRAGPFAAPKVPADVTQGVDREANGSVRYSYGKQHGENWAAHGPAPERYTWDE
TNFGSGRDTRDGFIQSAPLVVPPPLNGILTNANPIGTKNDIHFSNVFNSYGPLTAFSHPSPVYPQGQIWDKELDLEHKPR
LHITAPFVCKNNAPGQMLVRLGPNLTDQYDPNGATLSRIVTYGTFFWKGKLTMRAKLRANTTWNPVYQVSVEDNGNSYMS
VTKWLPTATGNMQSVPLITRPVARNTY