Solution structure of DNA binding domain A (DBD-A) of S.cerevisiae Replication Protein A (RPA)
Solution structure of the DNA-binding domain of RPA from Saccharomyces cerevisiae and its interaction with single-stranded DNA and SV40 T antigen
Replication Protein A, RF-A, Single-stranded DNA-binding protein, DNA binding protein BUF2, Replication protein A 69 kDa DNA- binding subunit
TRPIFAIEQLSPYQNVWTIKARVSYKGEIKTWHNQRGDGKLFNVNFLDTSGEIRATAFNDFATKFNEILQEGKVYYVSKA KLQPAKPQFTNLTHPYELNLDRDTVIEECFDESN
The crystal structure of the human homolog without DNA
The crystal structure of the complex of human homolog with DNA
The chemical shift assignments were done with triple-resonance experiments. Restraints were get from 3D 13C, 15N NOESY data.
The structure calculations were performed using 1138 interproton distance restraints and 161 dihedral angle restraints.
Torsional Angle Dynamics with Internal Variable Module
1mM scRPA DBD A U-15N, 13C; 20mM Sodium Phosphate, 100mM NaCl, 2mM DTT, pH 7.0; 90% H2O, 10% D2O
1mM scRPA DBD A U-15N; 20mM Sodium Phosphate, 100mM NaCl, 2mM DTT, pH 7.0; 90% H2O, 10% D2O