RDC-refined Solution NMR structure of reduced putidaredoxin
Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings.
A refined model for the solution structure of oxidized putidaredoxin
New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, ADX(4-108)
An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2Fe-2S Ferredoxin from pseudomonas
Redox-Dependent 1H NMR spectral features and tertiary structural constraints on the C-terminal region of putidaredoxin
SKVVYVSHDGTRRELDVADGVSLMQAAVSNGIYDIVGDCGGSASCATCHVYVNEAFTDKVPAANEREIGMLECVTAELKP NSRLCCQIIMTPELDGIVVDVPDRQW
The structures were refined against 15N-1H RDC restraints using previously published NOE and dihedral angle restraints for Pdx. The restraints for the metal binding loop (residues 36-48,85-86) were modeled from bovine adrenodoxin crystal structure.
15N-coupled 2D 15N-1H HSQC; 7.5% bicelle (DMPC:DHPC-2.9:1)
15N-coupled 2D 15N-1H HSQC; 10 mg/mL Phage
The structures are based on 1241 NOE restriants, 160 dihedral angle restraints and 150 RDC restraints, 28 paramagnetic distance restraints from relaxation measurements and 18 distance restraints from hydrogen bonds
1 mM reduced putidaredoxin 15N; 50 mM Tris-Cl buffer, 50 mM KCl, pH 7.4
ferredoxin, [2Fe-2S], redox, iron-sulfur, electron transfer, cytochrome P450cam, ELECTRON TRANSPORT