Phosphorylation of either Ser16 or Thr30 does not disrupt the structure of the Itch E3 ubiquitin ligase third WW domain
structures with the least restraint violations
The employed refinement engine was CNS 1.1 , but using the water refinement CNS scripts supplied with ARIA 2.0a
simulated annealing, torsion angle dynamics
Sodium Phosphate 20mM, Sodium Chloride 100mM