The solution structure of YGGX from Escherichia Coli
The solution structure of the oxidative stress-related protein YggX from Escherichia coli.
MGSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQHKQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKE VHIEGYTPEDKKLEHHHHHH
all calculated structures submitted, back calculated data agree with experimental NOESY spectrum, structures with acceptable covalent geometry, structures with favorable non-bond energy, structures with the least restraint violations, structures with the lowest energy, target function
THE STRUCTURES ARE BASED ON 2059 DISTANCE CONSTRAINTS, 72 DIHEDRAL ANGLE CONSTRAINTS AND 42 RESIDUAL DIPOLAR COUPLING RESTRAINTS. THE C-TERMINAL HIS TAG (RESIDUES 93-100) HAS BEEN REMOVED FORM THE COORDINATES.
Distance geometry, simulated annealing, molecular dynamics
0.8mM YggX U-15N,13C, 50mM phosphate, 150mM NaCl, 90% H2O, 10% D2O
0.8mM YggX U-15N,13C, 50mM phosphate, 150mM NaCl, 100% D2O
SRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQHKQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVH IEGYTPEDKK