DISTAL POCKET POLARITY IN LIGAND BINDING TO MYOGLOBIN: DEOXY AND CARBONMONOXY FORMS OF A THREONINE68 (E11) MUTANT INVESTIGATED BY X-RAY CRYSTALLOGRAPHY AND INFRARED SPECTROSCOPY

PHE A 151 - GLN A 152 OMEGA ANGLE = 146.397 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION

Distal pocket polarity in ligand binding to myoglobin: deoxy and carbonmonoxy forms of a threonine68(E11) mutant investigated by X-ray crystallography and infrared spectroscopy.

High Resolution X-Ray Structures of Pig Metmyoglobin and Two Cd3 Mutants Mb(Lys45-> Arg) and Mb(Lys45-> Ser)

Distal Polarity in Ligand Binding to Myoglobin: Structural and Functional Characterization of a Threonine68(E11) Mutant

Determination of the Crystal Structure of Recombinant Pig Myoglobin by Molecular Replacement and its Refinement

Apomyoglobin as a Molecular Recognition Surface: Expression, Reconstitution and Crystallisation of Recombinant Porcine Myoglobin in Escherichia Coli

GLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGNTTLTALGGILKKKG
HHEAELTPLAQSHATKHKIPVKYLEFISEAIIQVLQSKHPGDFGADAQGAMSKALELFRNDMAAKYKELGFQG

THE DATA ARE 72% COMPLETE. HOWEVER, ONLY 39% OF REFLECTIONS ARE PRESENT BETWEEN INFINITY AND 2DMIN. AS A RESULT THE ELECTRON DENSITY MAPS HAVE BREAKS PERIODICALLY ALONG THE MAIN CHAIN. ATOMS WHICH COULD NOT BE OBSERVED IN ANY MAP HAVE BEEN ASSIGNED AN OCCUPANCY OF 0.0. DURING THE COURSE OF REFINEMENT REASONABLY TIGHT RESTRAINTS WERE APPLIED TO THE GEOMETRY WHILE MOST ATTENTION WAS PAID TO THE HAEM GROUP AND ITS ENVIRONMENT. THIS STRATEGY WAS ADOPTED AS THE PURPOSE OF THE STUDY WAS TO DEFINE THE HAEM POCKET AND THE STEREOCHEMISTRY OF THE BOUND LIGAND.

GLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGNTVLTALGGILKKKG
HHEAELTPLAQSHATKHKIPVKYLEFISEAIIQVLQSKHPGDFGADAQGAMSKALELFRNDMAAKYKELGFQG