Crystal Structure of Mycobacterium tuberculosis NAD kinase-NAD complex

NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex

MTAHRSVLLVVHTGRDEATETARRVEKVLGDNKIALRVLSAEAVDRGSLHLAPDDMRAMGVEIEVVDADQHAADGCELVL
VLGGDGTFLRAAELARNASIPVLGVNLGRIGFLAEAEAEAIDAVLEHVVAQDYRVEDRLTLDVVVRQGGRIVNRGWALNE
VSLEKGPRLGVLGVVVEIDGRPVSAFGCDGVLVSTPTGSTAYAFSAGGPVLWPDLEAILVVPNNAHALFGRPMVTSPEAT
IAIEIEADGHDALVFCDGRREMLIPAGSRLEVTRCVTSVKWARLDSAPFTDRLVRKFRLPVTGWRGK

Ammonium sulfate, HEPES-Na, NAD, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

The biological unit is a tetramer generated from the dimer in the asymmetric unit by the operations: -x, -y, z.

NAD kinase, polyphosphate, NAD, Mycobacterium tuberculosis, TRANSFERASE