Crystal Structure of Xanthine Phosphoribosyltransferase from Bacillus subtilis.

Midwest Center for Structural Genomics (MCSG)

SNA(MSE)EALKRKIEEEGVVLSDQVLKVDSFLNHQIDPLL(MSE)QRIGDEFASRFAKDGITKIVTIESSGIAPAV
(MSE)TGLKLGVPVVFARKHKSLTLTDNLLTASVYSFTKQTESQIAVSGTHLSDQDHVLIIDDFLANGQAAHGLVSIVKQ
AGASIAGIGIVIEKSFQPGRDELVKLGYRVESLARIQSLEEGKVSFVQEVHS

SNAMEALKRKIEEEGVVLSDQVLKVDSFLNHQIDPLLMQRIGDEFASRFAKDGITKIVTIESSGIAPAVMTGLKLGVPVV
FARKHKSLTLTDNLLTASVYSFTKQTESQIAVSGTHLSDQDHVLIIDDFLANGQAAHGLVSIVKQAGASIAGIGIVIEKS
FQPGRDELVKLGYRVESLARIQSLEEGKVSFVQEVHS

Bis-tris, NaCl, PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

HETEROGEN
Ligand G4P co-purified and crystallized with the
protein. To explain the electron density, the
author modeled part of the naturally occurring
mononucleotide guanosine 3',5'-bis(diphosphate).
The 5' diphosphate was omitted because of poor
density.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Xanthine phosphoribosyltransferase (E.C.2.4.2.-)

xanthine, purine metabolism, phosphoribosyltransferase, structural genomics, PSI, Protein Structure Initative, Midwest Center for Structural Genomics, MCSG, Protein Structure Initiative, TRANSFERASE

MEALKRKIEEEGVVLSDQVLKVDSFLNHQIDPLLMQRIGDEFASRFAKDGITKIVTIESSGIAPAVMTGLKLGVPVVFAR
KHKSLTLTDNLLTASVYSFTKQTESQIAVSGTHLSDQDHVLIIDDFLANGQAAHGLVSIVKQAGASIAGIGIVIEKSFQP
GRDELVKLGYRVESLARIQSLEEGKVSFVQEVHS