Solution structure of a recombinant type I sculpin antifreeze protein
Type 1 antifreeze protein from Winter Flounder (4 degrees C)
Type 1 antifreeze protein from Winter Flounder (-180 degrees C)
This structure was determined using standard triple-resonance NMR spectroscopy.
Structure calculations were performed using the package ARIA 1.2. Final stuctures are based on 437 unambiguous restraints, 18 sets of ambiguous restraints, 46 dihedral angle restraints and 18 HN residual dipolar coupling restraints.
simulated annealing, molecular dynamics, torsion angle dynamics
1mM rSS3 U-15N,13C; pH adjusted to 5.0 using NaOH
type I antifreeze protein, sculpin, NMR spectroscopy, solution structure, a-helix, ANTIFREEZE PROTEIN