Solution structure of the second Ran-binding domain from human RanBP2
Solution structure of the Ran-binding domain 2 of RanBP2 and its interaction with the C terminus of Ran.
Sequence-specific resonance assignment of the second Ran-binding domain of human RanBP2
RanBP2, Nuclear pore complex protein Nup358, Nucleoporin Nup358, 358 kDa nucleoporin, P270
GSGEEDEKVLYSQRVKLFRFDAEVSQWKERGLGNLKILKNEVNGKLRMLMRREQVLKVCANHWITTTMNLKPLSGSDRAW MWLASDFSDGDAKLEQLAAKFKTPELAEEFKQKFEECQRLLLDIPLQTPK
The structures are based on a total of 1492 restraints (1281 NOE-derived distance restraints, 182 dihedral angle restraints and 29 hydrogen bond restraints). The structures were also refined in water using XPLOR-NIH 2.9.6 using the protocol of Linge et al. (Linge, J. P., Williams, M. A., Spronk, C. A., Bonvin, A. M., & Nilges, M. ,2003. Refinement of protein structures in explicit solvent. Proteins 50, 496-506).
1.4mM RanBD2 U-13C; U-15N; 150mM Na2SO4; 10mM DTE; 0.5 mM EDTA; 1mM NaN3; 0.1mM DSS; 10mM potassium phosphate buffer at pH 6.5;
0.7mM RanBD2 U-13C; U-15N; 150mM Na2SO4; 10mM DTE; 0.5mM EDTA; 1mM NaN3; 0.1mM DSS; 10mM potassium phosphate buffer at pH 6.5;
1.0mM RanBD2 U-15N; 150mM Na2SO4; 10mM DTE; 0.5mM EDTA; 1mM NaN3; 0.1 mM DSS; 10mM potassium phosphate buffer at pH 6.5;
1.0mM RanBD2 U-15N; 150mM Na2SO4; 10mM DTE; 0.5mM EDTA; 1mM NaN3; 0.1mM DSS; 10mM potassium phosphate buffer at pH 6.5;
beta barrel, pleckstrin-homology (PH) domain, phosphotyrosine-binding (PTB) domain, PROTEIN TRANSPORT
GEEDEKVLYSQRVKLFRFDAEVSQWKERGLGNLKILKNEVNGKLRMLMRREQVLKVCANHWITTTMNLKPLSGSDRAWMW LASDFSDGDAKLEQLAAKFKTPELAEEFKQKFEECQRLLLDIPLQTP