Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae
U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction
Solution Structure of the Shortened U6 ISL
Solution Structure Of Domain 5 From The Ai5(gamma) Group II Intron
Crystal Structure Of A RNA Molecule Containing Domain 5 and 6 Of The Yeast Ai5G Group II Self-Splicing Intron
In vitro transcription of U6 nucleotides 59-88 using purified T7 RNA polymerase and synthetic DNA oligonucleotides.
435 NOE distance constraints, 244 dihedral angle restraints, 66 hydrogen bond restraints, 77 residual dipolar couplings
simulated annealing, torsion angle dynamics, molecular dynamics, residual dipolar couplings, conformational database potentials
1mM extended U6 ISL RNA; 90% H2O; 10% D2O
1mM 13C,15N labeled extended U6 ISL RNA; 90% H2O; 10% D2O
1mM 13C,15N labeled extended U6 ISL RNA; 99.99% D2O
1mM 13C,15N labeled extended U6 ISL RNA; 17 mg/ml pf1 bacteriophage; 90% H2O; 10% D2O
U6 RNA, RNA stem-loop, penta-loop, internal bulge, metal binding site, RNA