Twenty Lowest Energy Structures of Pa4 by Solution NMR
Structure and orientation of pardaxin determined by NMR experiments in model membranes
Pardaxins naturally occur in sole fish of genus Pardachirus. Pa4 was synthesized using standard 9-fluorenylmethoxycarbonyl-based solid-pahse methods with an ABI 431A peptide synthesizer. Crude peptide was purified by reversed-phase HPLC
extended structure; random simulated annealing; molecular dynamics
~1.5 mg of lyophilized Pa4 in 20 mM PBS (pH 6.5),300 mM d31-DPC and 10% D2O. Sample pH was adjusted to ~4.5 with NaOH.
Bend-helix-bend-helix motif, SIGNALING PROTEIN