Alkaline M-protease form I crystal structure

High-resolution crystal structure of M-protease: phylogeny aided analysis of the high-alkaline adaptation mechanism

AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVL
GVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSIS
YPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGVAALVKQKNPSWSNVQI
RNHLKNTATGLGNTNLYGSGLVNAEAATR

acetate, ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

SUBTILISIN, DETERGENT ENZYME, HIGH-ALKALINE, HYDROLASE