Structure of aminopeptidase P from E. coli

Structural and functional implications of metal ion selection in aminopeptidase p, a metalloprotease with a dinuclear metal center

Aminopeptidase P, X-Pro aminopeptidase, Aminopeptidase P II, APP-II, Aminoacylproline aminopeptidase

SEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPYRQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRV
RDLTAEIWFGRRLGQDAAPEKLGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAPA
TMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHHEFNRHGARYPSYNTIVGSGENGC
ILHYTENE(CSO)EMRDGDLVLIDAGCEYKGYAGDITRTFPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGE
VVRIMVSGLVKLGILKGDVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPDAEVPE
QYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ

SEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPYRQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRV
RDLTAEIWFGRRLGQDAAPEKLGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAPA
TMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHHEFNRHGARYPSYNTIVGSGENGC
ILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRTFPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRI
MVSGLVKLGILKGDVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPDAEVPEQYRG
IGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ

Tris, PEG 4k, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Old refinement of hexagonal crystal form

Published hexagonal structure without Mn or solvent atoms

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The biological assembly is a homotetramer generated by the symmetry operatiors: (x,y,z), (1-x,1-y,z), (y,x,4/3-z), (1-y,1-x,4/3-z)

Proline-specific peptidase, metalloenzyme, pita-bread fold, hydrolase