Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2
Chemical synthesized 17-mer RNA corresponding to the RT binding site of eel LINE RNA.
The structures are based on a total of 298 restraints, 204 are NOE-derived distance constraints, 80 dihedral angle restraints, 14 distance restraints from hydrogen bonds.
1mM RNA FRAGMENT; 10mM phosphate buffer, 50 mM NaCl; 100% D2O
1mM RNA FRAGMENT; 10mM phosphate buffer, 50 mM NaCl; 90% H2O, 10% D2O
1mM RNA FRAGMENT (15N, 13C-Guanosine, Uridine labeled 36-mer RNA); 10mM phosphate buffer, 50 mM NaCl; 100% D2O
1mM RNA FRAGMENT (15N, 13C-Guanosine, Uridine labeled 36-mer RNA); 10mM phosphate buffer, 50 mM NaCl; 90% H2O, 10% D2O
RNA, pentaloop, LINE, retrotransposition