STREPTAVIDIN COMPLEXED WITH CYCLO-AC-[CHPQGPPC]-NH2 MONOMER, PH 2.5

In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.

Structure-Based Design Tools: Structural and Thermodynamic Comparison with Biotin of a Small Molecule that Binds Streptavidin with Micromolar Affinity

Preparation of a Protein-Dimerizing Ligand by Topochemistry and Structure-Based Design

Topochemical Catalysis Achieved by Structure-Based Ligand Design

Topochemistry for Preparing Ligands that Dimerize Receptors

Binding to Protein Targets of Peptidic Leads Discovered by Phage Display: Crystal Structures of Streptavidin-Bound Linear and Cyclic Peptide Ligands Containing the Hpq Sequence

Structure-Based Design of High Affinity Streptavidin Binding Cyclic Peptide Ligands Containing Thioether Cross-Links

AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTW
SGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKP

SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5.

THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: 13, 14, 15, GLN 24, (CG, OD1, AND OD2 OF ASP 36), ALA 46, VAL 47, GLY 48, ASN 49, ALA 50, SIDE CHAIN OF GLU 51, TERMINUS OF ARG 53, GLY 99, ALA 100, (CG, CD, OE1, OE2 OF GLU 101), (C, O OF GLU 101), TERMINUS OF ARG 103, (N, CA, CB, CG, OF GLN 116), (CD, OE1, OE2 OF GLN 116), ALA 117, (CG, CD, OF LYS 121), (CE, NZ OF LYS 121), (ACE P 0 AND CYS P 1), PRO P 7, (CYS P 8 AND NH2 P 9).

STREPTAVIDIN, PEPTIDE LIGAND CONTAINING HPQ

COMPLEX (BIOTIN-BINDING PROTEIN/PEPTIDE)
COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE), CYCLIC PEPTIDE DISCOVERED BY PHAGE DISPLAY, COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE) complex

MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVL
TGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPS
AASIDAAKKAGVNNGNPLDAVQQ