STREPTAVIDIN COMPLEXED WITH CYCLO-AC-[CHPQGPPC]-NH2 MONOMER, PH 2.5
In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.
Structure-Based Design Tools: Structural and Thermodynamic Comparison with Biotin of a Small Molecule that Binds Streptavidin with Micromolar Affinity
Preparation of a Protein-Dimerizing Ligand by Topochemistry and Structure-Based Design
Topochemical Catalysis Achieved by Structure-Based Ligand Design
Topochemistry for Preparing Ligands that Dimerize Receptors
Binding to Protein Targets of Peptidic Leads Discovered by Phage Display: Crystal Structures of Streptavidin-Bound Linear and Cyclic Peptide Ligands Containing the Hpq Sequence
Structure-Based Design of High Affinity Streptavidin Binding Cyclic Peptide Ligands Containing Thioether Cross-Links
AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTW SGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKP
SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.5.
THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: 13, 14, 15, GLN 24, (CG, OD1, AND OD2 OF ASP 36), ALA 46, VAL 47, GLY 48, ASN 49, ALA 50, SIDE CHAIN OF GLU 51, TERMINUS OF ARG 53, GLY 99, ALA 100, (CG, CD, OE1, OE2 OF GLU 101), (C, O OF GLU 101), TERMINUS OF ARG 103, (N, CA, CB, CG, OF GLN 116), (CD, OE1, OE2 OF GLN 116), ALA 117, (CG, CD, OF LYS 121), (CE, NZ OF LYS 121), (ACE P 0 AND CYS P 1), PRO P 7, (CYS P 8 AND NH2 P 9).
STREPTAVIDIN, PEPTIDE LIGAND CONTAINING HPQ
COMPLEX (BIOTIN-BINDING PROTEIN/PEPTIDE)
COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE), CYCLIC PEPTIDE DISCOVERED BY PHAGE DISPLAY, COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE) complex
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVL TGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPS AASIDAAKKAGVNNGNPLDAVQQ