STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 2.0
In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.
Structure-Based Design Tools: Structural and Thermodynamic Comparison with Biotin of a Small Molecule that Binds Streptavidin with Micromolar Affinity
Preparation of a Protein-Dimerizing Ligand by Topochemistry and Structure-Based Design
Topochemical Catalysis Achieved by Structure-Based Ligand Design
Topochemistry for Preparing Ligands that Dimerize Receptors
Binding to Protein Targets of Peptidic Leads Discovered by Phage Display: Crystal Structures of Streptavidin-Bound Linear and Cyclic Peptide Ligands Containing the Hpq Sequence
Structure-Based Design of High Affinity Streptavidin Binding Cyclic Peptide Ligands Containing Thioether Cross-Links
AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTW SGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKP
SYNTHETIC MOTHER LIQUOR = 75 % SATURATED AMMONIUM SULFATE, 25 % 1.0 M SODIUM FORMATE ADJUSTED TO PH 2.0.
THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: 13, 14, 15, SIDE CHAIN OF GLN 24, TERMINUS OF ASP 36, SIDE CHAIN OF 47, 48, 50, 51, TERMINUS OF ARG 53, 64, 65, 66, 67, 99, 100, SIDE CHAIN OF 101, TERMINUS OF ARG 103, TERMINUS OF GLU 116, TERMINUS OF LYS 121, (ACE P 0 AND CYS P 1), (CYS P 6 AND NH2 P 7). DISCRETELY DISORDERED SIDE CHAINS WHOSE OCCUPANCIES AND STRUCTURES WERE SIMULTANEOUSLY REFINED WERE 43, 84 (3 CONFORMATIONS), 107, 132. DISORDERED WATERS ARE HOH 207 WHICH IS CLOSE TO A SYMMETRY-RELATED EQUIVALENT OF HOH 354; HOH 244 WHICH IS CLOSE TO A SYMMETRY-RELATED EQUIVALENT OF HOH 298. HOH 305, HOH 313, AND HOH 467 ARE CLOSE TO SYMMETRY-RELATED EQUIVALENTS OF THEMSELVES, RESPECTIVELY. NO ENERGY INTERACTIONS INVOLVING HOH 305, HOH 313, OR HOH 467 WERE TURNED ON DURING REFINEMENT. THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: 13, 14, 15, SIDE CHAIN OF GLN 24, TERMINUS OF ASP 36, SIDE CHAIN OF 47, 48, 50, 51, TERMINUS OF ARG 53, 64, 65, 66, 67, 99, 100, SIDE CHAIN OF 101, TERMINUS OF ARG 103, TERMINUS OF GLU 116, TERMINUS OF LYS 121, (ACE P 0 AND CYS P 1), (CYS P 6 AND NH2 P 7).
STREPTAVIDIN, PEPTIDE LIGAND CONTAINING HPQ
COMPLEX (BIOTIN-BINDING PROTEIN/PEPTIDE)
COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE), CYCLIC PEPTIDE DISCOVERED BY PHAGE DISPLAY, COMPLEX (BIOTIN-BINDING PROTEIN-PEPTIDE) complex
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVL TGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPS AASIDAAKKAGVNNGNPLDAVQQ