Crystal structure of Putative asparaginyl hydroxylase (2636534) from Bacillus subtilis at 2.60 A resolution

Joint Center for Structural Genomics (JCSG)

(MSE)GSDKIHHHHHH(MSE)SAVTESVLESIISPVT(MSE)SEFLEEYWPVKPLVARGEVERFTSIPGFEKVRTLENVL
AIYNNPV(MSE)VVGDAVIEESEGITDRFLVSPAEALEWYEKGAALEFDFTDLFIPQVRRWIEKLKAELRLPAGTSSKAI
VYAAKNGGGFKAHFDAYTNLIFQIQGEKTWKLAKNENVSNP(MSE)QHYDLSEAPYYPDDLQSYWKGDPPKEDLPDAEIV
NLTPGT(MSE)LYLPRGLWHSTKSDQATLALNITFGQPAWLDL(MSE)LAALRKKLISDNRFRELAVNHQSLHESSKSEL
NGYLESLIQTLSENAETLTPEQIFQSQDSDFDPYQSTQLVFRQLLTSYKF

MGSDKIHHHHHHMSAVTESVLESIISPVTMSEFLEEYWPVKPLVARGEVERFTSIPGFEKVRTLENVLAIYNNPVMVVGD
AVIEESEGITDRFLVSPAEALEWYEKGAALEFDFTDLFIPQVRRWIEKLKAELRLPAGTSSKAIVYAAKNGGGFKAHFDA
YTNLIFQIQGEKTWKLAKNENVSNPMQHYDLSEAPYYPDDLQSYWKGDPPKEDLPDAEIVNLTPGTMLYLPRGLWHSTKS
DQATLALNITFGQPAWLDLMLAALRKKLISDNRFRELAVNHQSLHESSKSELNGYLESLIQTLSENAETLTPEQIFQSQD
SDFDPYQSTQLVFRQLLTSYKF

24% NP_PEG 4000, 0.065M Tris_base, 0.16M Mg Cl, 0.035M Tris Cl, 20% Glycerol, CuCl2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K

1.HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2.THE DENSITIES FOR THE FOLLOWING REGIONS, 66-96, 178-185, 268-275, 138-142, ARE VERY POOR. ALTHOUGH MODEL WAS BUILT FOR SOME OF THESE REGIONS, THEY MAY CONTAIN ERRORS. 3.A FERRIC IRON IS MODELLED FOR EACH CHAIN. THIS ASSIGNMENT WAS BASED ON HOMOLOGS (1IZ3 AND 1MZE). HOWEVER, THIS CRYSTAL WAS OBTAINED IN BUFFER CONTAINING COPPER. THERE IS NO EVIDENCE TO EXCLUED THE POSSIBILITY THE ION BEING OTHER METALS SUCH AS COPPER. 4.D278-D285 IS A PART OF HELIX, THE REFINEMENT OF THIS REGION IS NOT STABLE DUE TO THE POOR DENSITY.

2636534, putative asparaginyl hydroxylase, Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI, OXIDOREDUCTASE

MSAVTESVLESIISPVTMSEFLEEYWPVKPLVARGEVERFTSIPGFEKVRTLENVLAIYNNPVMVVGDAVIEESEGITDR
FLVSPAEALEWYEKGAALEFDFTDLFIPQVRRWIEKLKAELRLPAGTSSKAIVYAAKNGGGFKAHFDAYTNLIFQIQGEK
TWKLAKNENVSNPMQHYDLSEAPYYPDDLQSYWKGDPPKEDLPDAEIVNLTPGTMLYLPRGLWHSTKSDQATLALNITFG
QPAWLDLMLAALRKKLISDNRFRELAVNHQSLHESSKSELNGYLESLIQTLSENAETLTPEQIFQSQDSDFDPYQSTQLV
FRQLLTSYKF