Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima F132A/L128A/I123A mutant

A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products

MTKNKLNQNSYELEKVKERIEQILSQFFPEQIMKDLPLYGKMLRVRLSILSFKNRGVEIGEDAISSLAALELVHLASLLH
DDVIDGARFRRGKETINFMYGDKAAVAAGDLVLVSAFHTVEEAGNNKARRAALNVIGKMSEAELIEQLSRYKPITKEEYL
RIVEGKSGALFGLALQLPALLEGELGEDLYNLGVTIGTIYQMFDDIMDFAGMEKIGKDGFLDLKNGVASFPLVTAMEKFP
EARQMFENRDWSGLMSFMREKGILKECEETLKVLVKNVIIENSWLRDFVDGIFKIKISS

Hepes, Lithium sulfate, PEG 900, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

The same protein (F132A/L128A/I123A/D62A mutant)

trans-type prenyltransferase, thermophilic, TRANSFERASE

MTKNKLNQNSYELEKVKERIEQILSQFFPEQIMKDLPLYGKMLRVRLSILSFKNRGVEIGEDAISSLAALELVHLASLLH
DDVIDGARFRRGKETINFMYGDKAAVAAGDLVLVSAFHTVEEIGNNKLRRAFLNVIGKMSEAELIEQLSRYKPITKEEYL
RIVEGKSGALFGLALQLPALLEGELGEDLYNLGVTIGTIYQMFDDIMDFAGMEKIGKDGFLDLKNGVASFPLVTAMEKFP
EARQMFENRDWSGLMSFMREKGILKECEETLKVLVKNVIIENSWLRDFVDGIFKIKISS